Elucidating the role of glycosylation in maintaining health and in diseases

Drug Innovation
16:00HRS
27Oct2016
Location
Blue and Pink Lecture Hall, UMCU
Speaker
Prof Carlito Lebrilla

Carlito B. Lebrilla

Prof Carlito Lebrilla
Department of Chemistry, University of California

Abstract
Glycans are short carbohydrate chains that are present as modifications on proteins and lipids, and as free oligosaccharides in all organisms. They play key roles in defining the functions of proteins and lipids and are therefore involved in nearly every disease from infection to cancer. Despite their importance, our abilities to measure and determine precise structures have been limited due to the large structural heterogeneity and the complexity of the structures. For this reason, our understanding of glycoconjugates is not as advanced as that of other biopolymers. Our research has focused on developing advanced separation methods, accurate and structurally informative mass spectrometry techniques and automated informatics tools that now make rapid throughput profiling of glycans possible. These methods are used to determine the role of glycans in diseases allowing us to observe and characterize the glycan signatures of cancer and autoimmune diseases in tissues and in blood. Similarly, the cell membrane is composed of a glycan rich environment that mediates cellular interactions. Glycan profiling of cell membrane shows glycan changes during cellular transformation as cancer cells become differentiated and more metastatic. Glycan profiling can also elucidate the changes in glycosylation as host cells interact with microbes and provide clues regarding the role of glycans in host-microbe interactions.

CV
Dr. Lebrilla's laboratory group focuses on the development of methods for the identification and characterization of oligosaccharides and glycoconjugates found in biological samples, including glycolipids and glycoproteins. Oligosaccharides play a key role in important cellular processes such as recognition, adhesion and division. These compounds however have been difficult to analyze and characterize structurally. The problems include the large number of stereoisomers and possible linkage combinations. The laboratory is developing methods to overcome many of the difficulties associated with oligosaccharide analysis. They employ high performance mass spectrometry and modern chromotographic analysis specifically for oligosaccharides. The research group is currently focused on the glycomic and glycomic proteomic analysis of milk. They have recently characterized the free oligosaccharides in mammalian milk including human, bovine, procine, murine, and several primate species. They are now performing glycoproteomic studies on milk and examine changes in glycosylation of specific proteins. Dr. Carlito B. Lebrilla is a Distinguished Professor at the University of California, Davis in the Department of Chemistry and Biochemistry and Molecular Medicine in the School of Medicine. He received his BS degree from the University of California, Irvine and Ph.D. from the University of California, Berkeley. He was an Alexander von Humboldt Fellow and a NSF-NATO Fellow at the Technical University in Berlin. He returned to the UC Irvine as a President’s Fellow and has been at UC Davis since 1989.  He has served as Chair of the Chemistry Department. His research is in Analytical Chemistry, primarily mass spectrometry with applications to clinical glycomics and biofunctional food.  He has over 320 peer-reviewed publications.  He is also co-editor of Mass Spectrometry Reviews and has been on the editorial board of Molecular and Cellular Proteomics, Mass Spectrometry Reviews, Journal of American Society for Mass Spectrometry, European Mass Spectrometry, and International Journal of Mass Spectrometry.

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